Fig. 8. Storage leads to increased Syk-dependent RBC clearance post-transfusion. Fresh human CD59 transgenic murine RBCs or those stored in AS-1 for 72 h were treated with sub-lytic quantities of A.) VLY or B.) PLY ex vivo for 30 min followed by transfusion into healthy recipient WT mice. RBC clearance increases in in response to both toxins following storage. C-D.) Increased storage-induced RBC clearance was prevented by inhibition of Syk kinase. Shown is the ratio of toxi-treated:untreated RBCs. Results shown are from 10 transfused mice in each experiment. Mann-Whitney, ***p<0.001.